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crispick sgrna design tool  (Broad Institute Inc)

 
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    Broad Institute Inc crispick sgrna design tool
    Crispick Sgrna Design Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispick+sgrna+design+tool/pm40550560-353-28-32?v=Broad+Institute+Inc
    Average 90 stars, based on 1 article reviews
    crispick sgrna design tool - by Bioz Stars, 2026-08
    90/100 stars

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    sgrna design tool crispick  (Broad Institute Inc)
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    Broad Institute Inc sgrna design tool crispick
    HEK293 FRT cells were transfected with plasmids containing the genes coding for Cas9 and three furin <t>sgRNAs.</t> Lysates from nine surviving cell populations were evaluated for furin expression by western blot (panel A). The untreated parental (P) HEK293 FRT line is also shown for comparison. Cells from well 2 (boxed) were clonally selected by serial dilution in a 96-well plate. Lysates from four clones were evaluated for furin expression by western blot (panel B). Well 1 (boxed) was selected for further analysis.
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    https://www.bioz.com/product/crispick+sgrna+design+tool/bio_rxiv__2023__05__31__542721-40-0-12?v=Broad+Institute+Inc
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    HEK293 FRT cells were transfected with plasmids containing the genes coding for Cas9 and three furin sgRNAs. Lysates from nine surviving cell populations were evaluated for furin expression by western blot (panel A). The untreated parental (P) HEK293 FRT line is also shown for comparison. Cells from well 2 (boxed) were clonally selected by serial dilution in a 96-well plate. Lysates from four clones were evaluated for furin expression by western blot (panel B). Well 1 (boxed) was selected for further analysis.

    Journal: bioRxiv

    Article Title: A furin-deficient HEK293 cell line suggests a role for furin trafficking in the intoxication pathway of recombinant immunotoxins based on Pseudomonas exotoxin A

    doi: 10.1101/2023.05.31.542721

    Figure Lengend Snippet: HEK293 FRT cells were transfected with plasmids containing the genes coding for Cas9 and three furin sgRNAs. Lysates from nine surviving cell populations were evaluated for furin expression by western blot (panel A). The untreated parental (P) HEK293 FRT line is also shown for comparison. Cells from well 2 (boxed) were clonally selected by serial dilution in a 96-well plate. Lysates from four clones were evaluated for furin expression by western blot (panel B). Well 1 (boxed) was selected for further analysis.

    Article Snippet: Single guide RNAs (sgRNAs) to the furin gene were designed using the Broad Institute sgRNA design tool (CRISPick) , .

    Techniques: Transfection, Expressing, Western Blot, Comparison, Serial Dilution, Clone Assay