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Broad Institute Inc crispick sgrna design tool
Crispick Sgrna Design Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispick+sgrna+design+tool/crispick+sgrna+design+tool/pm40550560-353-28-32
Average 90 stars, based on 1 article reviews
crispick sgrna design tool - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

CRISPR:

Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling
Article Snippet: .. For CRISPR/Cas9 gene editing, sgRNAs targeting STING and p53 were designed using the CRISPick sgRNA design tool (Broad Institute) 59 , and cloned into the pMRI-Amt retroviral vector 60 . shRNAs or sgRNAs targeting a non-mammalian gene (Renilla luciferase) or human VEGF were used as controls. ..

Article Title: Serotonin receptor 5-HT 2A as a potential target for HCC immunotherapy.
Article Snippet: Protein bands were visualized on a ChemiDoc Imager (Bio- Rad) and relative protein levels were determined by band densitometry measurements using FIJI software (NIH). .. CRISPR/Cas9-mediated gene KO in mouse CD8 T cells by lentiviral transduction gRNA sequences targeting Htr2a (coding for 5- HT2A) were picked from a list generated by the online CRISPick sgRNA Design Tool (Broad Institute, Massachusetts, USA) and inserted into a custom pLKO- gRNA- Thy1.1 using sticky ends generated by BsmBI digestion. ..

Clone Assay:

Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling
Article Snippet: .. For CRISPR/Cas9 gene editing, sgRNAs targeting STING and p53 were designed using the CRISPick sgRNA design tool (Broad Institute) 59 , and cloned into the pMRI-Amt retroviral vector 60 . shRNAs or sgRNAs targeting a non-mammalian gene (Renilla luciferase) or human VEGF were used as controls. ..

Retroviral:

Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling
Article Snippet: .. For CRISPR/Cas9 gene editing, sgRNAs targeting STING and p53 were designed using the CRISPick sgRNA design tool (Broad Institute) 59 , and cloned into the pMRI-Amt retroviral vector 60 . shRNAs or sgRNAs targeting a non-mammalian gene (Renilla luciferase) or human VEGF were used as controls. ..

Plasmid Preparation:

Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling
Article Snippet: .. For CRISPR/Cas9 gene editing, sgRNAs targeting STING and p53 were designed using the CRISPick sgRNA design tool (Broad Institute) 59 , and cloned into the pMRI-Amt retroviral vector 60 . shRNAs or sgRNAs targeting a non-mammalian gene (Renilla luciferase) or human VEGF were used as controls. ..

Luciferase:

Article Title: The volume regulated anion channel LRRC8C suppresses T cell function by regulating cyclic dinucleotide transport and STING-p53 signaling
Article Snippet: .. For CRISPR/Cas9 gene editing, sgRNAs targeting STING and p53 were designed using the CRISPick sgRNA design tool (Broad Institute) 59 , and cloned into the pMRI-Amt retroviral vector 60 . shRNAs or sgRNAs targeting a non-mammalian gene (Renilla luciferase) or human VEGF were used as controls. ..

Generated:

Article Title: Serotonin receptor 5-HT2A as a potential target for HCC immunotherapy
Article Snippet: Protein bands were visualized on a ChemiDoc Imager (Bio-Rad) and relative protein levels were determined by band densitometry measurements using FIJI software (NIH). .. gRNA sequences targeting Htr2a (coding for 5-HT 2A ) were picked from a list generated by the online CRISPick sgRNA Design Tool (Broad Institute, Massachusetts, USA) and inserted into a custom pLKO-gRNA-Thy1.1 using sticky ends generated by BsmBI digestion. ..

Article Title: Serotonin receptor 5-HT 2A as a potential target for HCC immunotherapy.
Article Snippet: Protein bands were visualized on a ChemiDoc Imager (Bio- Rad) and relative protein levels were determined by band densitometry measurements using FIJI software (NIH). .. CRISPR/Cas9-mediated gene KO in mouse CD8 T cells by lentiviral transduction gRNA sequences targeting Htr2a (coding for 5- HT2A) were picked from a list generated by the online CRISPick sgRNA Design Tool (Broad Institute, Massachusetts, USA) and inserted into a custom pLKO- gRNA- Thy1.1 using sticky ends generated by BsmBI digestion. ..

other:

Article Title: CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling
Article Snippet: Guide RNA oligonucleotides (synthesized from Sigma-Aldrich) were cloned in the pLentiCRISPRv2 vector (Addgene; #52961) at the BsmBI cloning site.

Article Title: INPP5D regulates inflammasome activation in human microglia.
Article Snippet: The guide RNAs were ligated into plasmid backbone (pXPR_003, Addgene) and the sgRNA plasmid along with a plasmid containing Cas9 (pLX_311-Cas9, Addgene) were transfected into iPSCs using Lipofectamine 3000.

Article Title: INPP5D regulates inflammasome activation in human microglia
Article Snippet: Guide RNAs were designed using the Broad Institute CRISPick sgRNA design tool , .

Transduction:

Article Title: Serotonin receptor 5-HT 2A as a potential target for HCC immunotherapy.
Article Snippet: Protein bands were visualized on a ChemiDoc Imager (Bio- Rad) and relative protein levels were determined by band densitometry measurements using FIJI software (NIH). .. CRISPR/Cas9-mediated gene KO in mouse CD8 T cells by lentiviral transduction gRNA sequences targeting Htr2a (coding for 5- HT2A) were picked from a list generated by the online CRISPick sgRNA Design Tool (Broad Institute, Massachusetts, USA) and inserted into a custom pLKO- gRNA- Thy1.1 using sticky ends generated by BsmBI digestion. ..



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Broad Institute Inc crispick sgrna design tool
Crispick Sgrna Design Tool, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Broad Institute Inc sgrna design tool crispick
HEK293 FRT cells were transfected with plasmids containing the genes coding for Cas9 and three furin <t>sgRNAs.</t> Lysates from nine surviving cell populations were evaluated for furin expression by western blot (panel A). The untreated parental (P) HEK293 FRT line is also shown for comparison. Cells from well 2 (boxed) were clonally selected by serial dilution in a 96-well plate. Lysates from four clones were evaluated for furin expression by western blot (panel B). Well 1 (boxed) was selected for further analysis.
Sgrna Design Tool Crispick, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispick+sgrna+design+tool/crispick+sgrna+design+tool/bio_rxiv__2023__05__31__542721-40-0-12
Average 90 stars, based on 1 article reviews
sgrna design tool crispick - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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HEK293 FRT cells were transfected with plasmids containing the genes coding for Cas9 and three furin sgRNAs. Lysates from nine surviving cell populations were evaluated for furin expression by western blot (panel A). The untreated parental (P) HEK293 FRT line is also shown for comparison. Cells from well 2 (boxed) were clonally selected by serial dilution in a 96-well plate. Lysates from four clones were evaluated for furin expression by western blot (panel B). Well 1 (boxed) was selected for further analysis.

Journal: bioRxiv

Article Title: A furin-deficient HEK293 cell line suggests a role for furin trafficking in the intoxication pathway of recombinant immunotoxins based on Pseudomonas exotoxin A

doi: 10.1101/2023.05.31.542721

Figure Lengend Snippet: HEK293 FRT cells were transfected with plasmids containing the genes coding for Cas9 and three furin sgRNAs. Lysates from nine surviving cell populations were evaluated for furin expression by western blot (panel A). The untreated parental (P) HEK293 FRT line is also shown for comparison. Cells from well 2 (boxed) were clonally selected by serial dilution in a 96-well plate. Lysates from four clones were evaluated for furin expression by western blot (panel B). Well 1 (boxed) was selected for further analysis.

Article Snippet: Single guide RNAs (sgRNAs) to the furin gene were designed using the Broad Institute sgRNA design tool (CRISPick) , .

Techniques: Transfection, Expressing, Western Blot, Comparison, Serial Dilution, Clone Assay